ABSTRACT
Objective:Based on the methodology of network pharmacology, this study preliminarily predicted the major targets and pathways of Andrographis herba in the treatment of breast cancer. Methods:The active components of Andrographis herba were screened by Traditional Chinese Medicine Systems Pharmacology Database (TCMSP); breast cancer targets were predicted and screened by Genecard database platform and OMIM database platform; active ingredient-targets networks were built by Cytoscape (3.7.2) software, and protein interaction networks were built by String database platform. Finally, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed by using Bioconductor platform and R language. Results:A total of 24 active ingredients of Andrographis herba and 13 234 targets for breast cancer were obtained, involving 71 targets related to Andrographis herba. There were 51 common targets for Andrographis herba-breast cancer. The top five targets are TP53, IL6, JUN, CCND1, and ERS1. Andrographis herba-breast cancer KEGG-enriched pathways are estrogen signaling pathway, P53 signaling pathway, Notch signaling pathway, and Wnt signaling pathway. Conclusions:The TP53, ERS1, and CCND1 and other targets may be the key targets for Andrographis herba paniculata to treat breast cancer. It plays an anti-breast cancer effect by regulating the signal pathways such as the estrogen receptor pathway and the P53 signaling pathway. This paper provides a theoretical basis for further exploration of the mechanism research of Andrographis herba in the treatment of breast cancer.
ABSTRACT
Objective:By taking network pharmacology approach to search for active components and the rapeutic targets of Huanglian-Ejiao Decoction. Methods:According to the pharmacokinetic parameters, this research screened components of this decoction, and diged the drugs and disease targets with network pharmacology and constructed the drug-target-pathway network by Cytoscape system to investigate the mechanism of Huanglian-Ejiao Decoction. Results:It was found that 19 components such as hydroberberine, β-sitosterol, curcumin, kaempferol, berberine and Ramie flavonoids in Huanglian-Ejiao Decoction could take effecton insomnia by regulating 25 targets such as 5-hydroxytryptamine, dopamine, gamma aminobutyric acid, opioid receptor and acetylcholine. Conclusion:Huanglian-Ejiao Decoction may play an important role in treating insomnia by regulating multiple-targets.